Which stem cell culture medium is right for you?
| Medium |
|
NutriStem® hPSC XF Medium, Modified GF-Free |
MSC NutriStem® XF Medium |
|||
| Cell Type | Human PSCs | Human PSCs | Human PSCs | Human PSCs | Human PSCs | Human MSCs |
| Application | Feeder-Dependent hPSC culture | Feeder-Free hPSC culture |
Translational hPSC culture | Translational hPSC culture, single cell | Reprogramming | Completely Defined Translational hMSC culture |
| Additional Supplement | None | None | None | None | bFGF (20 ng/mL) | MSC NutriStem® Supplement Mix |
| Matrix | MEFs/HFFs | Matrigel Cultrex Geltrex Other |
LaminStem™ 521 Laminin-511 |
LaminStem™ 521 | LaminStem™ 521 Matrigel Laminin-511 |
|
| Xeno-Free | Possible (HFFs) | No | Yes | Yes | Yes | Yes |
| Weekend-Free Feeding*> | No | Yes | Yes | Yes | n/a | n/a |
| Dissociation Reagent | Collagenase IV | Dispase Collagenase IV Accutase 0.5mM EDTA |
0.5mM EDTA Accutase |
Trypsin-EDTA Accutase |
Trypsin-EDTA Accutase |
Recombinant Trypsin Solution |
| Freezing Media | NutriFreez® D10 Cryopreservation Medium |
NutriFreez® D10 Cryopreservation Medium |
NutriFreez® D10 Cryopreservation Medium |
NutriFreez® D10 Cryopreservation Medium |
NutriFreez® D10 Cryopreservation Medium |
NutriFreez® D10 Cryopreservation Medium |
| Additional Reagents | Gelatin, Human Serum Albumin (HSA), Soybean Trypsin Inhibitor, EZ-PCR™ Mycoplasma Detection Kit, DPBS, Aquaguard™-1 Solution, Aquaguard™-2 Solution for Water Baths | |||||
*Weekend-free feeding is possible, though with any hPSC culture, optimal growth is seen with daily feeding. It is not recommended to skip feedings on a routine or long-term basis.
