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NutriCoat™ Attachment Solution

Cost-effective, efficient, standardized supplement designed for the attachment of human mesenchymal stem cells under serum-free and xeno-free culture conditions.

Temporarily out of stock

SKU: 05-760-1-15

Description

Details

Product Overview:

NutriCoat™ is a defined substrate based on clinical-grade human Fibrinogen, designed for the attachment of human mesenchymal stem cells (hMSC) in serum-free and xeno-free culture systems. NutriCoat™ Attachment Solution is optimal for adherence of hMSC’s from multiple sources (e.g. AT, CT, BM, DP) when cells are cultured with MSC NutriStem® XF Medium.

The substrate supports long term culturing of hMSC, as well as their enumeration using the colony forming unit-fibroblast (CFU-F) assay under SF and XF culture conditions. NutriCoat™ is a ready-to-use solution store at RT and ideal for cell therapy research applications.

Features

  • Defined substrate containing clinical grade human Fibrinogen (xeno-free)
  • Easy-to-use stock solution for easy handling
  • cGMP Manufactured
  • Suitable for both hMSC expansion and differentiation
  • Supports long-term multi-potency of hMSC
  • Validated for multiple sources of human MSC culture
  • Allows quick transition from research to clinical applications


Figure: Optimal growth in serum-free conditions. hMSC were cultured in MSC NutriStem® XF on plates coated with NutriCoat™. hMSC maintained typical fibroblast-like cells morphology.

Specifications

Specifications

QTY 1.5 mL/vial
Form Liquid
Brand NutriCoat™
Storage Conditions 15-30˚C
quality control Each lot is tested for sterility as well as its biological performance (the ability to promote the attachment and spreading of hMSC in serum-free medium).
Instructions for Use

Instructions for Use

  1. Dilute the solution 1:500 in saline and gently mix using a pipette.
  2. Coat the culture dish with the 1:500 solution. The volume should be adequate for covering the surface of the culture ware. Use table 1 for the recommended volumes. (e.g. per 1 well of 12w/p add 2μl of NutriCoat™ in 1 ml saline)
  3. Gently agitate and incubate for 1 hour in a humidified CO2 incubator (37°C)
  4. Prior to cells seeding, carefully discard the solution and add culture medium
Culture Vessel Surface area (cm2) 

Volume of 1:500

NutriCoat™  Attachment Solution

 96 well  0.34  0.1 mL
24 well  1.9  0.5 mL
 12 well  3.9  1 mL
 6 well
 9.6  2.5 mL
 T25 Flask   25  6.5 mL
 T75 Flask   75  19 mL

 

Important Notes 

  1. Longer incubation of coated culture dish (up to 24 hours) will not affect cell attachment and spreading
  2. One wash with Dulbecco’s PBS (without Ca & Mg) is an option
  3. It is critical that the coating does not dry out

Documentation

Certificate of Analysis

Certificates of Analysis (COA's) describe quality control data for specific product lots.

COA's can be downloaded from Sartorius's Certificates Portal.

For certificates issued before November 15, 2021, please enter below the product lot number and click search.
If your search does not return any results, please contact us for assistance.

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