Description
Details
Lymphocyte Separation Tubes
EZ Lympho-Sep™ is used as an alternative to the "home made" blood separation tube.Density gradient centrifugation of whole blood on a polysucrose – sodium metrizoate medium is the method of choice for isolation of lymphocytes.
The success of the procedure, i.e. the recovery of viable lymphocytes with the lowest proportion of contaminating granulocytes and erythrocytes, depends to a large extent on the careful layering of the blood sample onto the separation medium and the maintenance of a sharp interface between the two solutions prior to centrifugation.
The EZ Lympho-Sep™ system allows the blood sample to be poured directly into the centrifuge tube with no special precautions required to prevent disruption of the polysucrose - sodium metrizoate layer. Thus, a large number of samples may be handled at the same time. The mechanism also reduces the length of centrifugation time required for separation of the lymphocytes.
The heart of the EZ Lympho-Sep™ is a plastic insert that allows the blood sample to be poured directly into the tube alleviating the need for slow and careful addition of the blood. Secondly, a one-way feature of the insert allows passage of materials during the centrifugation step but prevents the flow of the separation medium during shipping. After centrifugation, the upper lymphocyte-containing fraction may be poured off without risk of contamination from the erythrocytes, which are trapped under the insert.
EZ Lympho-Sep™ are ready-to-use sterile tubes with the separation medium already in place, and the one-way valve opening during centrifugation.
EZ Lympho-Sep™ Features
- Safe method, minimum contact with biological fluids
- Time saver, quick and easy sample filling
- Maximum yield of viable mononuclear cells
- A large number of samples may be handled at the same time
Specifications
Specifications
| Storage Conditions | 4-25°C |
|---|
Documentation
Manuals and Protocols
Product Sheet
Certificate of Analysis
Certificates of Analysis (COA's) describe quality control data for specific product lots.
COA's can be downloaded from Sartorius's Certificates Portal.
For certificates issued before November 15, 2021, please enter below the product lot number and click search.
COA's can be downloaded from Sartorius's Certificates Portal.
For certificates issued before November 15, 2021, please enter below the product lot number and click search.
If your search does not return any results, please contact us for assistance.
